Abstract Mycotoxin contamination in stored sorghum grains poses significant threats to food safety, human health, and agricultural economies in sub-Saharan Africa, particularly in Ethiopia where poor post-harvest practices exacerbate fungal proliferation. This study, conducted in Dejen District, East Gojjam Zone, Ethiopia, aimed to assess mycotoxin contamination levels, farmers' knowledge and management practices, isolate and characterize mycotoxigenic fungi, and evaluate the efficacy of microbial antagonists and botanicals as eco-friendly control measures. A cross-sectional survey of 212 farmers revealed low awareness (29% good knowledge) and practices (40.57% effective management), with significant associations to age, education, and village location (p < 0.05, logistic regression). Mycological analysis of 120 stored sorghum samples from underground pits identified diverse fungi, dominated by Aspergillus flavus (25%) and Fusarium spp. (20%), with low mycotoxin levels (aflatoxin B1 at 1.05 µg/kg, below EU limits). Six fungal antagonists; four Trichoderma (DMUA13, DMUA14) and two Penicillium isolates were isolated and screened via dual-culture assays, achieving 44.53–75.00% inhibition of radial growth against Aspergillus and Fusarium spp. (p < 0.05). Ethanol extracts of Clematis simensis and Laggera tomentosa leaves demonstrated dose-dependent antifungal activity against A. niger, reducing spore germination by up to 68.58% and 68.32% at 100 µg/mL, respectively, comparable to ketoconazole. These findings underscore knowledge gaps among farmers and highlight the potential of native Trichoderma/Penicillium isolates and plant extracts as sustainable biocontrol agents. Targeted education, improved storage, and integration of biological controls are recommended to mitigate mycotoxin risks, enhancing food security in resource-limited settings. This research provides baseline data for policy interventions and further field validation.

Abstract Mycotoxin contamination in stored sorghum grains poses significant threats to food safety, human health, and agricultural economies in sub-Saharan Africa, particularly in Ethiopia where poor post-harvest practices exacerbate fungal proliferation. This study, conducted in Dejen District, East Gojjam Zone, Ethiopia, aimed to assess mycotoxin contamination levels, farmers' knowledge and management practices, isolate and characterize mycotoxigenic fungi, and evaluate the efficacy of microbial antagonists and botanicals as eco-friendly control measures. A cross-sectional survey of 212 farmers revealed low awareness (29% good knowledge) and practices (40.57% effective management), with significant associations to age, education, and village location (p < 0.05, logistic regression). Mycological analysis of 120 stored sorghum samples from underground pits identified diverse fungi, dominated by Aspergillus flavus (25%) and Fusarium spp. (20%), with low mycotoxin levels (aflatoxin B1 at 1.05 µg/kg, below EU limits). Six fungal antagonists; four Trichoderma (DMUA13, DMUA14) and two Penicillium isolates were isolated and screened via dual-culture assays, achieving 44.53–75.00% inhibition of radial growth against Aspergillus and Fusarium spp. (p < 0.05). Ethanol extracts of Clematis simensis and Laggera tomentosa leaves demonstrated dose-dependent antifungal activity against A. niger, reducing spore germination by up to 68.58% and 68.32% at 100 µg/mL, respectively, comparable to ketoconazole. These findings underscore knowledge gaps among farmers and highlight the potential of native Trichoderma/Penicillium isolates and plant extracts as sustainable biocontrol agents. Targeted education, improved storage, and integration of biological controls are recommended to mitigate mycotoxin risks, enhancing food security in resource-limited settings. This research provides baseline data for policy interventions and further field validation.

Foodborne illnesses as a result of poor food handling practicespose a significant threat to public health. The main objective of thissystematic review and meta-analysis was to pool the level ofhygienic food handling practices among food handlers working inpublic food establishments in the Amhara region, Ethiopia. Aninclusive search of databases was undertaken usingPubMed/MEDLINE, SCOPUS, Web of Science, and GoogleScholar from the 1st of January 2014 to the 30th of December 2023.Data was collected, entered into Excel, and finally exported toStata V.17 for analysis. Eyeball testing using forest plots, CochraneQ test statistics and I2 had been used to identify and measure het-erogeneity. The pooled prevalence of hygienic food handling prac-tices was estimated using a random effects model. The pooledprevalence of hygienic food handling practices of food handlers inthe Amhara region was 48% [95% confidence interval (CI): (43%,53%)] with significant heterogeneity (I2=94.39%, p

Objectives: This study aimed to assess the prevalence and risk factors of malaria in individuals visiting health centers in the wetlands of Bahir Dar Zuria District, Northwest Ethiopia.
Methods: A cross-sectional study design was used on 422 study participants from three selected health centers from February to March 2020. Plasmodium parasite detection was examined using thick and thin blood smears, with a rapid diagnostic test. The chi-square (χ2) test was used to analyze the association between the prevalence of malaria and categorical variables, whereas binary logistic regression was used to identify potential risk factors using SPSS Version 25. For all types of tests, P-values below 0.05 were considered significant.
Results: The overall prevalence of malaria observed at the selected health centers around the wetlands of Bahir Dar Zuria District was 23.90% (95% confidence interval: 20.30-27.70%). There was a higher prevalence of malaria in males (39.78%) than in females (11.44%). The younger age group (16-30 years) was the most affected (47.76%), compared with the remaining 0-2 (19.23%), 3-15 (8.64%), 36-45 (37.25%), and above 45 years age groups (13.01%). Individuals with bed net-using habit decreased the risk of malaria infection by 99.70% compared with their counterparts (adjusted odds ratio: 0.003, 95% confidence interval: 0.001-0.011).
Conclusions: In this study, the wetlands of Bahir Dar Zuria District were found conducive to the survival and reproduction of mosquitoes, and the occurrence of malaria prevalence was very high. Therefore, wetland management practices and community mobilizations that might decrease vector abundance and malaria transmission should be strengthened in the study area.

Keywords: Bahir Dar Zuria; Malaria; Prevalence; Rapid diagnostic test; Wetlands.

Objectives: This study aimed to assess the prevalence and risk factors of intestinal schistosomiasis in students atBochesa Elementary School in the wetlands of Lake Ziway.Methods: A cross-sectional study of 384 students was conducted in May 2016. Intestinal schistosomiasis wasexamined using the Kato-Katz technique. For the association between the prevalence of intestinal schistosomiasisand categorical variables, the chi-square (𝜒2 ) test was used. A binary logistic regression was applied.Results: The overall prevalence of intestinal schistosomiasis was 25.52%. Males were more infected (17.45%)than women (8.07%), and the difference was significant (𝜒2 = 17.756; P

Dry evergreen Afromontane forests are severely threatened due to the expansion of agriculture and overgrazing by livestock. The objective of this study was to investigate the composition of woody species, structure, regeneration status and plant communities in Seqela forest, as well as the relationship between plant community types and environmental variables. Systematic sampling was used to collect vegetation and environmental data from 52 (20 m x 20 m) (400 m2) plots. Density, Diameter at Breast Height (DBH), basal area, frequency, and importance value index (IVI) of woody species were computed to characterize the vegetation structure of the forest. Agglomerative hierarchical cluster analysis and Canonical Correspondence Analysis (CCA) with R software were used to identify plant communities and analyse the relationship between plant community types and environmental variables, respectively. A total of 68 woody plant species belonging to 63 genera and 44 families were identified. The Shannon diversity index and evenness values of the study area were 2.12 and 0.92, respectively. The total basal area and density of woody species were 27.4 m2 ha-1 and 1079.3 individual ha-1, respectively. The most frequent woody species in the Seqela forest included Albizia gummifera (51.92%), Croton macrostachyus (44.23%), Olinia rochetiana and Teclea nobilis (36.54%). Additionally, the most dominant species, as indicated by their importance value index (IVI), were Erythrina brucei (IVI = 11.24), Prunus africana (IVI=8.68), and Croton macrostachyus (IVI=7.38). Four plant community types were identified: Albizia gummifera - Ekebergia capensis, Prunus africana - Croton macrostachyus, Vachellia abyssinica - Dombeya torrida and Schefflera abyssinica - Teclea nobilis. The CCA results showed that the variation of species distribution and plant community formation were significantly (P < 0.05) related to altitude, organic matter, aspect, slope and soil available phosphorus. The regeneration status assessment of the forest revealed a good regeneration status, which was linked to diverse and abundant seed bank in the soil can ensure a continuous supply of seeds for regeneration; therefore, it is recommended to implement periodic soil seed bank assessments to monitor seed diversity and abundance and inform targeted conservation actions.

Abstract: Plants are rich sources of phytochemicals, secondary metabolites like alkaloids, terpenoids, steroids, flavonoids, saponins, phenolics, glycosides, and others, found in various plant parts including leaves, fruits, roots, and stem bark. This study aimed to investigate the phytochemical components of Ruta chalepensis, Vernonia amygdalina, andZehneria scabraspecies in Senan District, Northwestern Ethiopia. Methanol and petroleum ether extracts of selected plant parts were subjected to standard qualitative phytochemical screening. The analysis revealed the presence of several phytochemical compounds in both methanol and petroleum ether extracts of the aerial parts of R. chalepensisand the leaves of V. amygdalinaand Z. scabra. Notably, methanolic extracts exhibited a higher abundance of phytochemicals due to the superior solubility of active compounds compared to petroleum ether. These findings highlight the potential of these plants as valuable sources of therapeutic agents. Traditionally, R. chalepensis, V. amygdalina, and Z. scabrahave been employed to treat various ailments. While this study provides a foundation for further exploration, additional research, including quantitative analysis, compound characterization, isolation, and pharmacological evaluation, is necessary to fully unlock these plants' therapeutic potential. Keywords:Ethnobotany,Medicinalplants,Phytochemicalscreening,Secondarybioactivechemicals

This study evaluated the antibacterial activity of medicinalplants (MPs) used to treat wounds. Methanol and ethyl acetatecrude extracts of four medicinal plants were examined for anti-bacterial efficacy against gram-positive and gram-negativeAmerican Type Culture Collection (ATCC) strains. Plant crudeextracts were produced using the maceration technique andanalyzed using qualitative phytochemical tests. The antibacter-ial properties of plant products were evaluated using the agardisc diffusion assay. The broth microdilution method was usedto determine the minimum inhibitory concentrations (MIC) andminimum bactericidal concentrations. The data were analyzedusing Analysis of Variance (ANOVA). The methanol extract ofRumex nervosus had the highest yield (21%). The methanolextracts of all tested MPs tested positive for alkaloids, phenolics,and tannins. The methanol and ethyl acetate extracts exhibiteda dose-dependent increase in the growth inhibition zoneagainst all ATCC. The methanol extract of Plantago lanceolatademonstrated the highest antibacterial activity (16.67 ± 1.15)against Staphylococcus epidermidis. S. aureus and S. epidermidisrecorded a relatively lower MIC (6.25 mg mL−1) from methanolextracts of P. lanceolata, while P. lanceolata and R. nervosusrecorded a lower MBC (25 mg mL−1) against S. aureus.
KEYWORDS: Antibacterial activity; inhibition zone; medicinal plants; MIC; phytochemical; wound

Abstract: Understanding how land use and land cover (LULC) changes affect soil erosion is essentialfor effective management of watershed areas. This study used Geographic Information Systems(GISs) and the Revised Universal Soil Loss Equation (RUSLE) model to analyze the impact of LULCchanges on soil erosion in the Dondor Watershed. Remote sensing data, including Landsat andSentinel-2 satellite images, alongside field surveys, topographic data, rainfall, and soil data wereused. The results showed agricultural land as the primary LULC type, increasing from 43.49% in2002 to 59.10% in 2023. Forest and built-up areas also expanded, while grassland decreased. Soilerosion estimates revealed that more than 85% of the watershed experienced very slight erosionthough the average annual soil loss increased from 4.98 t ha−1 year−1 in 2002 to 7.96 t ha−1 year−1in 2023. Agriculture and built-up areas were identified as the primary contributors to erosion. Thisstudy underscores the importance of monitoring LULC dynamics for responsible land managementand conservation efforts in the watershed.Keywords: Dondor watershed; land use land cover change; soil erosion; RUSLE

O. abyssinica, known in Ethiopia as lowland bamboo, is a solid-stemmed clump-forming bamboo species widelydistributed in the western dry regions of Ethiopia. The versatility of the species means that it has enormouspotential for land restoration in arid and semi-arid areas, in addition to bringing socio-economic benefits. It alsodisplays remarkable adaptability, allowing it to thrive in the challenging ecological conditions of areas such asthe Abay Gorge. The Abay Gorge, situated within the Upper Blue Nile River Basin of Northwestern Ethiopia ischaracterized by problems associated with soil erosion and land degradation. This study, therefore, aimed toevaluate and recommend a suitable spatial analysis for the adoption and development of lowland bamboo(O. abyssinica). Fifteen primary influencing factors were chosen according to the needs of O. abyssinica, theaccessibility of data, and the financial implications associated with data analysis, particularly the expensesrelated to soil laboratory testing. Eight composite soil samples were taken directly by dividing the watershed intotwo categories (upper and lower catchment areas). These composite soil samples were analyzed. Data analysiswas performed via Analytical Hierarchy Process (AHP) in conjunction with Multi-Criteria Decision-Making(MCDM) analysis and the use of Remote Sensing (RS) and Geographic Information System (GIS). The spatialanalysis employed in this study was a weighted sum overlay analysis, which was applied by considering thecriteria weight assigned to each factor. The analysis revealed that 21.2 % (666.5 ha) of the total area was highlysuitable for the growth and development of O. abyssinica. About 56 % (1753 ha) of the land was moderately andmarginally suitable for the desired land utilization type. However, 23 % (723.8 ha) of the study area was un-suitable for O. abyssinica. O. abyssinica has the potential to rehabilitate large parts of challenging areas such as theAbay Gorge due to its naturally invase rhizomes and drought-tolerance. It can also be introduced into agrofor-estry systems and used as living fences for villages.
Keywords: AHP, GIS, Land evaluation, Lowland bamboo, MCDM, Remote sensing