Debre Markos University, located in Ethiopia, maintains an Institutional Research Repository System that stores, manages, and distributes digital research outputs such as theses, dissertations, and other scholarly works. This system helps preserve academic work and makes it accessible to researchers, students, and the community.
Search Results (221 found)
Application of Precision Agriculture Technologies for Sustainable Crop Production and Environmental Sustainability: A Systematic Review
Sewnet Getahun, Habtamu Kefale, and Yohannes Gelaye ()
College of Agriculture and Natural resourcesPlant Science
Abstract Preview:
Precision agriculture technologies (PATs) transform crop production by enabling more sustainable and efcient agriculturalpractices. Tese technologies utilize data-driven approaches to optimize the management of crops, soil, and resources, thusenhancing both productivity and environmental sustainability. Tis article reviewed the application of PATs for sustainable cropproduction and environmental sustainability around the globe. Key components of PAT include remote sensing, GPS-guidedequipment, variable rate technology (VRT), and Internet of Tings (IoT) devices. Remote sensing and drones deliver high-resolution imagery and data, enabling precise monitoring of crop health, soil conditions, and pest activity. GPS-guided machineryensures accurate planting, fertilizing, and harvesting, which reduces waste and enhances efciency. VRToptimizes resource use byallowing farmers to apply inputs such as water, fertilizers, and pesticides at varying rates across a feld based on real-time data andspecifc crop requirements. Tis reduces over-application and minimizes environmental impact, such as nutrient runof andgreenhouse gas emissions. IoT devices and sensors provide continuous monitoring of environmental conditions and crop status,enabling timely and informed decision-making. Te application of PAT contributes signifcantly to environmental sustainabilityby promoting practices that conserve water, reduce chemical usage, and enhance soil health. By enhancing the precision ofagricultural operations, these technologies reduce the environmental impact of farming, while simultaneously boosting cropyields and proftability. As the global demand for food increases, precision agriculture ofers a promising pathway to achievingsustainable crop production and ensuring long-term environmental health.Keywords: environmental sustainability; precision agriculture technologies; precision farming; sustainable crop production
Full Abstract:
Precision agriculture technologies (PATs) transform crop production by enabling more sustainable and efcient agriculturalpractices. Tese technologies utilize data-driven approaches to optimize the management of crops, soil, and resources, thusenhancing both productivity and environmental sustainability. Tis article reviewed the application of PATs for sustainable cropproduction and environmental sustainability around the globe. Key components of PAT include remote sensing, GPS-guidedequipment, variable rate technology (VRT), and Internet of Tings (IoT) devices. Remote sensing and drones deliver high-resolution imagery and data, enabling precise monitoring of crop health, soil conditions, and pest activity. GPS-guided machineryensures accurate planting, fertilizing, and harvesting, which reduces waste and enhances efciency. VRToptimizes resource use byallowing farmers to apply inputs such as water, fertilizers, and pesticides at varying rates across a feld based on real-time data andspecifc crop requirements. Tis reduces over-application and minimizes environmental impact, such as nutrient runof andgreenhouse gas emissions. IoT devices and sensors provide continuous monitoring of environmental conditions and crop status,enabling timely and informed decision-making. Te application of PAT contributes signifcantly to environmental sustainabilityby promoting practices that conserve water, reduce chemical usage, and enhance soil health. By enhancing the precision ofagricultural operations, these technologies reduce the environmental impact of farming, while simultaneously boosting cropyields and proftability. As the global demand for food increases, precision agriculture ofers a promising pathway to achievingsustainable crop production and ensuring long-term environmental health.Keywords: environmental sustainability; precision agriculture technologies; precision farming; sustainable crop production
The effect of seed and nitrogen phosphorous fertilizer rates on growth and yield components of bread wheat (Triticum aestivum L.) in Burie District, Northwestern Ethiopia
College of Agriculture and Natural resourcesPlant Science
Abstract Preview:
In the absence of a recommended optimal seed rate and nitrogen-phosphorous (NP) fertilizers application for a spe-cific area, achieving maximum bread wheat productivity becomes challenging. To address this issue, this field experi-ment was conducted to evaluate the effect of seed and NP fertilizer rates on growth, yield, and yield components ofbread wheat (Triticum aestivum L.). Factorial combinations of four levels of seed rates of bread wheat (100, 120, 150, and200 kg ha−1 ) and four levels of NP fertilizers rates (64–46, 87–46, 96–69, and 119–69 kg ha−1 of N and P 2 O 5 ) werelaid down in randomized complete block design with three replications. The remaining necessary agronomic practicesand crop management activities were undertaken uniformly. The data presented in this dataset article includes pheno-logical, growth, yield, and yield component parameters that were collected timely following their standard methods andprocedures. All the collected data were subjected to analysis of variance (ANOVA) which was carried out using theSAS version 9.0 software computer program’s General Linear Model (GLM) procedure [1]. the residuals were evalu-ated, as described in Montgomery [2], to confirm the normal distribution and homogeneous variance model assump-tions on the error terms for each response variable. The independence assumption is upheld due to the randomization ofthe sixteen (16) treatment combinations within each block. In cases where a treatment effect was found to be significant,a multiple means comparison was conducted at a 5% significance level using Fisher’s LSD method to create letter group-ings. Additionally, correlation analysis was carried out using the Pearson correlation procedure in SAS. This dataset articleprovides insights into how seed rate and NP fertilizer rates impact bread wheat productivity, as well as the economic viability of optimal seed rate and NP fertilizer rates on bread wheat productivity. The information presented can serve as a valuable resource for researchers looking to examine thesupplementary data and methods in detail, potentially lead ing to new research avenues. Furthermore, it has the potential to foster collaborations and enhance the credibility of the current research data within the scientific community, making it accessible for wider use.
Keywords: Bread wheat, Burie district, Dataset, Grain yield, Seed rate, NP fertilizers, Partial budget analysis
Full Abstract:
In the absence of a recommended optimal seed rate and nitrogen-phosphorous (NP) fertilizers application for a spe-cific area, achieving maximum bread wheat productivity becomes challenging. To address this issue, this field experi-ment was conducted to evaluate the effect of seed and NP fertilizer rates on growth, yield, and yield components ofbread wheat (Triticum aestivum L.). Factorial combinations of four levels of seed rates of bread wheat (100, 120, 150, and200 kg ha−1 ) and four levels of NP fertilizers rates (64–46, 87–46, 96–69, and 119–69 kg ha−1 of N and P 2 O 5 ) werelaid down in randomized complete block design with three replications. The remaining necessary agronomic practicesand crop management activities were undertaken uniformly. The data presented in this dataset article includes pheno-logical, growth, yield, and yield component parameters that were collected timely following their standard methods andprocedures. All the collected data were subjected to analysis of variance (ANOVA) which was carried out using theSAS version 9.0 software computer program’s General Linear Model (GLM) procedure [1]. the residuals were evalu-ated, as described in Montgomery [2], to confirm the normal distribution and homogeneous variance model assump-tions on the error terms for each response variable. The independence assumption is upheld due to the randomization ofthe sixteen (16) treatment combinations within each block. In cases where a treatment effect was found to be significant,a multiple means comparison was conducted at a 5% significance level using Fisher’s LSD method to create letter group-ings. Additionally, correlation analysis was carried out using the Pearson correlation procedure in SAS. This dataset articleprovides insights into how seed rate and NP fertilizer rates impact bread wheat productivity, as well as the economic viability of optimal seed rate and NP fertilizer rates on bread wheat productivity. The information presented can serve as a valuable resource for researchers looking to examine thesupplementary data and methods in detail, potentially lead ing to new research avenues. Furthermore, it has the potential to foster collaborations and enhance the credibility of the current research data within the scientific community, making it accessible for wider use.
Keywords: Bread wheat, Burie district, Dataset, Grain yield, Seed rate, NP fertilizers, Partial budget analysis
College of Agriculture and Natural resourcesVeterinary laboratory Technology
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Knowledge of domestic dog ecology and demography has been recognized as central to the designof an effective rabies control program. The study was conducted to assess owned dogs’ ecologyand demography and to identify predictors associated with dog ownership and rabies occurrencein the Amhara region, Ethiopia.Method: ology: The study employed dog census and questionnaire surveys of 907 householdsselected using a multistage sampling technique from six rural and six urban districts of theAmhara region, Ethiopia. The ecology and demography of owned dogs in the selected areas wererecorded and described using descriptive statistics. Mixed-effect logistic regression models wereused to identify factors associated with dog ownership and rabies occurrence.Results: A total of 6609 dogs were estimated from 42 kebeles in the 12 study districts. The male-to-female ratio of dogs was 1.7:1.0, and the mean age of dogs was 3.2 years. The proportion ofhouseholds who owned at least one dog was 5.9 %. The average number of dogs per dog-ownedhousehold was 1.3. Dog to household ratio was 1.0:13.0, and dog to human ratio was 1.0:48.5.The majority of the dog owners (97 %) keep dogs for home guard and livestock herding. Only 57% of the dogs were confined, and 16 % of them were vaccinated. Ninety-one percent of the dogowners did not practice neutering and spaying for dog population control. Religion, livestockownership pattern, and occupation were associated with dog ownership (p < 0.05). Communityresidence and age of respondents were associated with rabies occurrence (p < 0.05), while zonewas associated with both dog ownership and rabies occurrence at p-value
Full Abstract:
Knowledge of domestic dog ecology and demography has been recognized as central to the designof an effective rabies control program. The study was conducted to assess owned dogs’ ecologyand demography and to identify predictors associated with dog ownership and rabies occurrencein the Amhara region, Ethiopia.Method: ology: The study employed dog census and questionnaire surveys of 907 householdsselected using a multistage sampling technique from six rural and six urban districts of theAmhara region, Ethiopia. The ecology and demography of owned dogs in the selected areas wererecorded and described using descriptive statistics. Mixed-effect logistic regression models wereused to identify factors associated with dog ownership and rabies occurrence.Results: A total of 6609 dogs were estimated from 42 kebeles in the 12 study districts. The male-to-female ratio of dogs was 1.7:1.0, and the mean age of dogs was 3.2 years. The proportion ofhouseholds who owned at least one dog was 5.9 %. The average number of dogs per dog-ownedhousehold was 1.3. Dog to household ratio was 1.0:13.0, and dog to human ratio was 1.0:48.5.The majority of the dog owners (97 %) keep dogs for home guard and livestock herding. Only 57% of the dogs were confined, and 16 % of them were vaccinated. Ninety-one percent of the dogowners did not practice neutering and spaying for dog population control. Religion, livestockownership pattern, and occupation were associated with dog ownership (p < 0.05). Communityresidence and age of respondents were associated with rabies occurrence (p < 0.05), while zonewas associated with both dog ownership and rabies occurrence at p-value
College of Agriculture and Natural resourcesVeterinary laboratory Technology
Abstract Preview:
This study aims and documents the clinical and laboratory diagnosis of bovine dermatophilosis, caused by Dermatophiluscongolensis that causes exudation and matting of hairs and wools with the formation of crusts and scab. An adult local breed bullwith a history of reduced appetite, pruritus, dirty scabs and crusts as initial lesion coalesce on its different body parts was presentedto Debre Markos multipurpose veterinary clinic. The rectal temperature and other vital parameters were within the normalrange. The clinical signs observed were exudative dermatitis forming crusts and scabs, pruritus, matted hair like a paintbrushand keratinized material creating wart-like lesions that were widely distributed in different body parts. Few ticks were observedon the skin of the bull suspected as vector of the disease. Culture of skin scraps on blood agar showed small, grayish-white raisedgranular and hemolytic colonies. Staining of skin scraps by Giemsa and Gram’s stain and cultured colony by Gram’s stain indicatedthe characteristics of Dermatophilus congolensis, etiology of dermatophilosis. Other laboratory tests also showed the characteristicsof D. congolensis. The case was treated by penstrep and ivermectin parentally and iodine tincture topically, and the bull showedprogressive clinical improvement and complete recovery within 2 months. In conclusion, dermatophilosis is an economicallyimportant skin disease, highly prevalent in Ethiopia, and needs early detection and treatment by penstrep intramuscularly andivermectine subcutaneously with iodine tincture topically in line with proper husbandry practices to control the losses.
Keywords: Dermatophilus congolensis; bull; clinical diagnosis; dermatophilosis; skin.
Full Abstract:
This study aims and documents the clinical and laboratory diagnosis of bovine dermatophilosis, caused by Dermatophiluscongolensis that causes exudation and matting of hairs and wools with the formation of crusts and scab. An adult local breed bullwith a history of reduced appetite, pruritus, dirty scabs and crusts as initial lesion coalesce on its different body parts was presentedto Debre Markos multipurpose veterinary clinic. The rectal temperature and other vital parameters were within the normalrange. The clinical signs observed were exudative dermatitis forming crusts and scabs, pruritus, matted hair like a paintbrushand keratinized material creating wart-like lesions that were widely distributed in different body parts. Few ticks were observedon the skin of the bull suspected as vector of the disease. Culture of skin scraps on blood agar showed small, grayish-white raisedgranular and hemolytic colonies. Staining of skin scraps by Giemsa and Gram’s stain and cultured colony by Gram’s stain indicatedthe characteristics of Dermatophilus congolensis, etiology of dermatophilosis. Other laboratory tests also showed the characteristicsof D. congolensis. The case was treated by penstrep and ivermectin parentally and iodine tincture topically, and the bull showedprogressive clinical improvement and complete recovery within 2 months. In conclusion, dermatophilosis is an economicallyimportant skin disease, highly prevalent in Ethiopia, and needs early detection and treatment by penstrep intramuscularly andivermectine subcutaneously with iodine tincture topically in line with proper husbandry practices to control the losses.
Keywords: Dermatophilus congolensis; bull; clinical diagnosis; dermatophilosis; skin.
College of Agriculture and Natural resourcesVeterinary laboratory Technology
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Illnesses associated with consuming infected milk and milk products are a widespread problem inlow and middle-income countries. Shiga toxin-producing Escherichia coli (STEC) is a bacteriumcommonly found in raw milk and causes foodborne diseases ranging from mild diarrhea to severehemorrhagic colitis and hemolytic uremic syndrome. This study aimed to investigate the viru-lence gene and antimicrobial resistance profiles of Shiga toxin-producing E. coli strains isolatedfrom raw milk in dairy farms in and around Bahir Dar town. Raw milk samples (n = 128)collected from December 2021 to July 2022 were cultured, and E. coli strains were isolated usingstandard methods. Shiga toxin-producing E. coli strains were identified genotypically by thepresence of the virulence markers using a single-plex polymerase chain reaction. The antibioticsusceptibility testing of Shiga toxin-producing E. coli isolates was done by the agar disk diffusionmethod. In total, 32 E. coli isolates were recovered from milk samples from lactating animals. PCRscreening of these isolates resulted in 19 (59.3%) positives for Shiga toxin-producing E. coli. Thestx2 gene was detected in 53% of cases, followed by stx1 (31%) and eae (16%. The STEC isolateswere highly sensitive to ciprofloxacin (94.7%) and kanamycin (89.5%), while exhibiting signif-icant resistance to amoxicillin (89.5%) and streptomycin (73.7%). The present study points outthe occurrence of virulent and antibiotic-resistant Shiga toxin-producing E. coli strains in raw milkthat could pose a potential risk to public health. Further analysis by whole genome sequencing isnecessary for an in-depth assessment and understanding of their virulence and resistance factors.Moreover, large-scale studies are needed to identify the prevalence and potential risk factors andto prevent the spread of antibiotic-resistant STEC strains in the milk production chain.
Full Abstract:
Illnesses associated with consuming infected milk and milk products are a widespread problem inlow and middle-income countries. Shiga toxin-producing Escherichia coli (STEC) is a bacteriumcommonly found in raw milk and causes foodborne diseases ranging from mild diarrhea to severehemorrhagic colitis and hemolytic uremic syndrome. This study aimed to investigate the viru-lence gene and antimicrobial resistance profiles of Shiga toxin-producing E. coli strains isolatedfrom raw milk in dairy farms in and around Bahir Dar town. Raw milk samples (n = 128)collected from December 2021 to July 2022 were cultured, and E. coli strains were isolated usingstandard methods. Shiga toxin-producing E. coli strains were identified genotypically by thepresence of the virulence markers using a single-plex polymerase chain reaction. The antibioticsusceptibility testing of Shiga toxin-producing E. coli isolates was done by the agar disk diffusionmethod. In total, 32 E. coli isolates were recovered from milk samples from lactating animals. PCRscreening of these isolates resulted in 19 (59.3%) positives for Shiga toxin-producing E. coli. Thestx2 gene was detected in 53% of cases, followed by stx1 (31%) and eae (16%. The STEC isolateswere highly sensitive to ciprofloxacin (94.7%) and kanamycin (89.5%), while exhibiting signif-icant resistance to amoxicillin (89.5%) and streptomycin (73.7%). The present study points outthe occurrence of virulent and antibiotic-resistant Shiga toxin-producing E. coli strains in raw milkthat could pose a potential risk to public health. Further analysis by whole genome sequencing isnecessary for an in-depth assessment and understanding of their virulence and resistance factors.Moreover, large-scale studies are needed to identify the prevalence and potential risk factors andto prevent the spread of antibiotic-resistant STEC strains in the milk production chain.
College of Agriculture and Natural resourcesVeterinary laboratory Technology
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Abstract: Diarrheagenic Escherichia coli (DEC) are the leading cause of infectious diarrhea and posea significant global, regional, and national burden of disease. This study aimed to investigate theprevalence of six DEC pathotypes in children with diarrhea and determine their antibiotic resistancepatterns. Samples from 107 diarrheagenic children were collected and processed for Escherichiacoli (E. coli). Single-plex PCR was used to detect target virulence genes as well as characterize andcategorize DEC pathotypes. Antibiotic resistance patterns were determined by the Kirby–Bauerdisk diffusion method. E. coli was detected in 79 diarrheal stool samples, accounting for 73.8% ofthe samples collected. Additionally, 49.4% (39 out of 79) of the isolates harbored various typicalvirulence factors. Results revealed six pathotypes of virulence: enterotoxigenic E. coli (ETEC) (53.8%),enteropathogenic E. coli (EPEC) (12.8%), enteroaggregative E. coli (EAEC) (10.3%), Heteropathotypes(7.8%), Shiga toxin-producing E. coli (STEC), and enterohemorrhagic E. coli (EHEC) (7.7% each).The isolates exhibited high antibiotic resistance against trimethoprim/sulfamethoxazole (82.1%),amoxicillin (79.5%), ampicillin (74.4%), gentamicin (69.2%), and streptomycin (64.1%). An overalloccurrence of 84.6% of multiple-drug resistance was observed in the isolates, with resistance rangingfrom three to four antibiotic classes. Our findings revealed a high level of pathogenic E. coli that werehighly resistant to multiple categories of antibiotics among children in the Awi zone. These findingshighlight the potential role of pathogenic E. coli in childhood diarrhea in tropical low-resourcesettings and underscore the need for continued research on the characteristics of pathogenic andantibiotic-resistant strains.Keywords: children; diarrheagenic Escherichia coli; resistance; virulent genes; Awi zone
Full Abstract:
Abstract: Diarrheagenic Escherichia coli (DEC) are the leading cause of infectious diarrhea and posea significant global, regional, and national burden of disease. This study aimed to investigate theprevalence of six DEC pathotypes in children with diarrhea and determine their antibiotic resistancepatterns. Samples from 107 diarrheagenic children were collected and processed for Escherichiacoli (E. coli). Single-plex PCR was used to detect target virulence genes as well as characterize andcategorize DEC pathotypes. Antibiotic resistance patterns were determined by the Kirby–Bauerdisk diffusion method. E. coli was detected in 79 diarrheal stool samples, accounting for 73.8% ofthe samples collected. Additionally, 49.4% (39 out of 79) of the isolates harbored various typicalvirulence factors. Results revealed six pathotypes of virulence: enterotoxigenic E. coli (ETEC) (53.8%),enteropathogenic E. coli (EPEC) (12.8%), enteroaggregative E. coli (EAEC) (10.3%), Heteropathotypes(7.8%), Shiga toxin-producing E. coli (STEC), and enterohemorrhagic E. coli (EHEC) (7.7% each).The isolates exhibited high antibiotic resistance against trimethoprim/sulfamethoxazole (82.1%),amoxicillin (79.5%), ampicillin (74.4%), gentamicin (69.2%), and streptomycin (64.1%). An overalloccurrence of 84.6% of multiple-drug resistance was observed in the isolates, with resistance rangingfrom three to four antibiotic classes. Our findings revealed a high level of pathogenic E. coli that werehighly resistant to multiple categories of antibiotics among children in the Awi zone. These findingshighlight the potential role of pathogenic E. coli in childhood diarrhea in tropical low-resourcesettings and underscore the need for continued research on the characteristics of pathogenic andantibiotic-resistant strains.Keywords: children; diarrheagenic Escherichia coli; resistance; virulent genes; Awi zone
Occurrence of Methicillin-Resistant Staphylococcus aureus (MRSA) in Bovine Bulk Milk and Farm Workers in Smallholder Dairy Farming Systems in Northwestern Ethiopia
College of Agriculture and Natural resourcesVeterinary laboratory Technology
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Background and Purpose: Staphylococcus aureus is a common pathogen responsible for causing various human and animalinfections and is well known for its ability to develop resistance to multiple antibiotics. This study aimed to evaluate the occurrence ofmethicillin-resistant Staphylococcus aureus (MRSA) in bulk milk and dairy farms in northwestern Ethiopia and to determine theirphenotypic and genotypic antimicrobial susceptibility patterns.Methods: We collected 50 bulk milk samples from 50 dairy farms and 50 hand swabs from dairy milkers. The cefoxitin disk diffusiontest and PCR-based assays were used to identify MRSA isolates. In addition, cefoxitin-resistant isolates were tested for susceptibilityto other antibiotics using the Kirby-Bauer disk diffusion method.Results: The results showed that MRSA was detected in 8 samples: 6 from bulk milk samples (12%) and 2 from hand swabs (4%). AllMRSA isolates exhibited a high resistance rate to penicillin (100%), followed by tetracycline (75%), ciprofloxacin (25%), chloram-phenicol (25%), erythromycin (25%), gentamycin (12.5%), and trimethoprim-sulfamethoxazole (12.5%). Moreover, 72% of theisolates showed resistance to three or more antibiotic classes and were classified as multidrug-resistant.Conclusion: This study identified methicillin-resistant Staphylococcus aureus and multidrug-resistant MRSA in bulk milk and dairyfarms in northwestern Ethiopia. These findings highlight the potential risk of transmission of these antibiotic-resistant bacteria tohumans and the need for improved antibiotic stewardship in the dairy sector using the One Health approach.Keywords: dairy farms, Ethiopia, humans, MRSA, bulk milk, resistance
Full Abstract:
Background and Purpose: Staphylococcus aureus is a common pathogen responsible for causing various human and animalinfections and is well known for its ability to develop resistance to multiple antibiotics. This study aimed to evaluate the occurrence ofmethicillin-resistant Staphylococcus aureus (MRSA) in bulk milk and dairy farms in northwestern Ethiopia and to determine theirphenotypic and genotypic antimicrobial susceptibility patterns.Methods: We collected 50 bulk milk samples from 50 dairy farms and 50 hand swabs from dairy milkers. The cefoxitin disk diffusiontest and PCR-based assays were used to identify MRSA isolates. In addition, cefoxitin-resistant isolates were tested for susceptibilityto other antibiotics using the Kirby-Bauer disk diffusion method.Results: The results showed that MRSA was detected in 8 samples: 6 from bulk milk samples (12%) and 2 from hand swabs (4%). AllMRSA isolates exhibited a high resistance rate to penicillin (100%), followed by tetracycline (75%), ciprofloxacin (25%), chloram-phenicol (25%), erythromycin (25%), gentamycin (12.5%), and trimethoprim-sulfamethoxazole (12.5%). Moreover, 72% of theisolates showed resistance to three or more antibiotic classes and were classified as multidrug-resistant.Conclusion: This study identified methicillin-resistant Staphylococcus aureus and multidrug-resistant MRSA in bulk milk and dairyfarms in northwestern Ethiopia. These findings highlight the potential risk of transmission of these antibiotic-resistant bacteria tohumans and the need for improved antibiotic stewardship in the dairy sector using the One Health approach.Keywords: dairy farms, Ethiopia, humans, MRSA, bulk milk, resistance
Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective.
College of Agriculture and Natural resourcesVeterinary laboratory Technology
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Background Diarrheagenic Escherichia coli (E. coli) is a zoonotic pathogen that contaminates abattoir workers,slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with E. coliis associated with the consumption of contaminated food and water, and it is a potential threat to the healthand welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic E. coli and assess itsantibiogram profile in two abattoir settings, in one health lens.Methods A cross-sectional study in one health approach was conducted from December 2020 to June 2021. Atotal of 384 samples from abattoir workers’ hands, carcasses, knives, cattle feces, abattoir water and effluents werecollected. Bacterial culture and biochemical tests were conducted to isolate E. coli, while conventional polymerasechain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic E. coli was tested againstnine antimicrobials using the Kirby Bauer disk diffusion method.Results A total of 115 (29.95%) E. coli were isolated from the 384 samples, and from these isolates, about 17 (14.8%)were confirmed to be diarrheagenic E. coli (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three(17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic E. coli, respectively. While 14 (82.35%)DEC isolates harbored the stx2 gene, five (29.41%) the eae gene, five (29.41%) the hlyA gene and three (17.65%)harbored the st gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they weresusceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to bothceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance.Conclusion This study detected DEC isolates having different virulence genes, which showed single and multipleantimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food
chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health riskfrom the pathogen and antimicrobial resistance.Keywords Abattoir setting, Antibiogram profile, Carcasses, Diarrheagenic E. Coli, Virulence genes
Full Abstract:
Background Diarrheagenic Escherichia coli (E. coli) is a zoonotic pathogen that contaminates abattoir workers,slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with E. coliis associated with the consumption of contaminated food and water, and it is a potential threat to the healthand welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic E. coli and assess itsantibiogram profile in two abattoir settings, in one health lens.Methods A cross-sectional study in one health approach was conducted from December 2020 to June 2021. Atotal of 384 samples from abattoir workers’ hands, carcasses, knives, cattle feces, abattoir water and effluents werecollected. Bacterial culture and biochemical tests were conducted to isolate E. coli, while conventional polymerasechain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic E. coli was tested againstnine antimicrobials using the Kirby Bauer disk diffusion method.Results A total of 115 (29.95%) E. coli were isolated from the 384 samples, and from these isolates, about 17 (14.8%)were confirmed to be diarrheagenic E. coli (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three(17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic E. coli, respectively. While 14 (82.35%)DEC isolates harbored the stx2 gene, five (29.41%) the eae gene, five (29.41%) the hlyA gene and three (17.65%)harbored the st gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they weresusceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to bothceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance.Conclusion This study detected DEC isolates having different virulence genes, which showed single and multipleantimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food
chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health riskfrom the pathogen and antimicrobial resistance.Keywords Abattoir setting, Antibiogram profile, Carcasses, Diarrheagenic E. Coli, Virulence genes
College of Agriculture and Natural resourcesNatural Resource Management
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Small scale irrigation (SSI) technologies offer a promising solution to address challenges of low agricultural productivity, unreliable rainfall, and rural poverty in Ethiopia. However, their performance and sustainability remain inconsistent, particularly in regions like East Gojjam Zone, Amhara Region. This study aimed to assess the adoption trends, performance challenges, and economic viability of furrow irrigation and motor pump systems across two districts Debre Elias and Machakel. A cross sectional, mixedmethods design was employed, drawing on data from 280 smallholder farmers selected through a multistage sampling procedure. Descriptive analysis showed moderate irrigation access, but a 25.4% overall defect rate raised concerns about technology reliability. Binary logistic regression identified income, credit access, and training as key factors reducing defect occurrence, while motor pump use and larger irrigated areas increased risk. Economic analysis revealed that furrow systems were more profitable, yielding a higher five year Net Present Value and Benefit Cost Ratio than motor pumps. Despite these gains, sustainability challenges persisted, including soil degradation and inefficient water use. The study also found notable district level variation, with furrow systems dominating in Debre Elias due to affordability, and topography favoring gravity flow and motor pumps preferred in Machakel for their productivity potential. Qualitative findings highlighted high maintenance costs and limited institutional support as barriers to sustained use. Based on these insights, the study recommends strengthening farmer access to credit, improving training services, and upgrading irrigation technologies to enhance the long term impact of SSI on smallholder livelihoods and agricultural sustainability
Full Abstract:
Small scale irrigation (SSI) technologies offer a promising solution to address challenges of low agricultural productivity, unreliable rainfall, and rural poverty in Ethiopia. However, their performance and sustainability remain inconsistent, particularly in regions like East Gojjam Zone, Amhara Region. This study aimed to assess the adoption trends, performance challenges, and economic viability of furrow irrigation and motor pump systems across two districts Debre Elias and Machakel. A cross sectional, mixedmethods design was employed, drawing on data from 280 smallholder farmers selected through a multistage sampling procedure. Descriptive analysis showed moderate irrigation access, but a 25.4% overall defect rate raised concerns about technology reliability. Binary logistic regression identified income, credit access, and training as key factors reducing defect occurrence, while motor pump use and larger irrigated areas increased risk. Economic analysis revealed that furrow systems were more profitable, yielding a higher five year Net Present Value and Benefit Cost Ratio than motor pumps. Despite these gains, sustainability challenges persisted, including soil degradation and inefficient water use. The study also found notable district level variation, with furrow systems dominating in Debre Elias due to affordability, and topography favoring gravity flow and motor pumps preferred in Machakel for their productivity potential. Qualitative findings highlighted high maintenance costs and limited institutional support as barriers to sustained use. Based on these insights, the study recommends strengthening farmer access to credit, improving training services, and upgrading irrigation technologies to enhance the long term impact of SSI on smallholder livelihoods and agricultural sustainability
Modification of Biomass cooking Stove to Sustain the Environment and Better Health: Comparative Study on Conventional and improved biomass cooking Stove Efficiency (the case of East Gojjam, Ethiopia).
Dereje Gebrie, Mulatu Mengistayehu, Senait Negussie, Mikael Betseha and Megabit Beyene ()
College of Agriculture and Natural resourcesNatural Resource Management
Abstract Preview:
This study investigates the status of biomass stove usage in East Gojjam, Ethiopia, and evaluates the health, economic, and environmental benefits of an improved biomass stove which was designed and fabricated using locally available materials. Major challenges in the study areas were uses of traditional three-stone stoves, which are inefficient and produce harmful emissions. The study aims to assess the status of stoves used, source, amount and type of biomass consumption per household, to analyze the health impact of improved biomass stove with conventional stove and to analyze the economic and environmental impact of the conventional and improved stove.Two stove models were fabricated: one for household use and another for institutional settings. Construction materials included angle iron, sheet metal, ceramic tiles, sand, and clay for insulation. Eucalyptus wood a commonly used local biomass fuel was standardized for all testing, including oven drying and storage procedures. Performance evaluation was based on cooking time, fuel consumption, and emissions (CO and PM2.5), using laboratory and field tests. Additional data were collected through surveys, interviews, focus group discussions, and direct observations from 400 households across three districts: Dejen, Yedwuha and Debrework.According to the result; the improved biomass stove significantly outperformed the conventional three stone stove: reducing cooking time by 51.61%, fuel consumption by 12.34%, carbon monoxide emissions by 99.72%, and PM2.5 emissions by 75.52%. It also demonstrated substantial environmental benefits, including lower firewood demand and reduced deforestation. Economically, households saved over 3,225 birr per year in fuel and time costs.In conclusion, the improved biomass stove offers a viable, scalable solution to Ethiopia’s household energy and health challenges. Its adoption can significantly mitigate household air pollution, improve public health, reduce environmental degradation, and strengthen economic resilience, particularly for women and children. The study recommends expanding the dissemination of the improved biomass stoves through targeted policy support, community education, and financing mechanisms.
Full Abstract:
This study investigates the status of biomass stove usage in East Gojjam, Ethiopia, and evaluates the health, economic, and environmental benefits of an improved biomass stove which was designed and fabricated using locally available materials. Major challenges in the study areas were uses of traditional three-stone stoves, which are inefficient and produce harmful emissions. The study aims to assess the status of stoves used, source, amount and type of biomass consumption per household, to analyze the health impact of improved biomass stove with conventional stove and to analyze the economic and environmental impact of the conventional and improved stove.Two stove models were fabricated: one for household use and another for institutional settings. Construction materials included angle iron, sheet metal, ceramic tiles, sand, and clay for insulation. Eucalyptus wood a commonly used local biomass fuel was standardized for all testing, including oven drying and storage procedures. Performance evaluation was based on cooking time, fuel consumption, and emissions (CO and PM2.5), using laboratory and field tests. Additional data were collected through surveys, interviews, focus group discussions, and direct observations from 400 households across three districts: Dejen, Yedwuha and Debrework.According to the result; the improved biomass stove significantly outperformed the conventional three stone stove: reducing cooking time by 51.61%, fuel consumption by 12.34%, carbon monoxide emissions by 99.72%, and PM2.5 emissions by 75.52%. It also demonstrated substantial environmental benefits, including lower firewood demand and reduced deforestation. Economically, households saved over 3,225 birr per year in fuel and time costs.In conclusion, the improved biomass stove offers a viable, scalable solution to Ethiopia’s household energy and health challenges. Its adoption can significantly mitigate household air pollution, improve public health, reduce environmental degradation, and strengthen economic resilience, particularly for women and children. The study recommends expanding the dissemination of the improved biomass stoves through targeted policy support, community education, and financing mechanisms.